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sample_name:"DNA-directed RNA Polymerase I complex and RNA and DNA"

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Current Database

  • EMDB [2]

Current status

  • REL [2]

Sample type

  • Complex [2]
  • DNA [2]
  • Ligand [2]
  • Protein [2]
  • RNA [2]

Organism

  • Saccharomyces cerevisiae [2]

EM Method

  • Single-particle [2]

Resolution

  • ≥ 2Å and < 3Å [1]
  • ≥ 3Å and < 4Å [1]

Release Date

  • 2025 [2]

Author by name

  • Fernandez-tornero C [2]
  • Gil-carton D [2]
  • Hou P [2]
  • Plaza-pegueroles A [2]
  • Ruiz FM [2]
  • Santos-aledo A [2]
  • Sanz-murillo M [2]
  • Wang D [2]
  • Xu J [2]

Journal

  • NAT COMMUN [2]

Software

  • CRYOLO [1]
  • CRYOSPARC [1]
  • CTFFIND [1]
  • GCTF [1]
  • RELION [1]

Microscope

  • FEI TITAN KRIOS [2]

Electron Source

  • FIELD EMISSION GUN [2]

Film or detector model

  • GATAN K2 SUMMIT (4K X 4K) [2]
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EMD-50955
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3D View
Yeast RNA polymerase I elongation complex stalled by an apurinic site
Release date: June 18, 2025
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 9g1v
Q-score: 0.549

Santos-Aledo A, Plaza-Pegueroles A, Sanz-Murillo M, Ruiz FM, Hou P, Xu J, Gil-Carton D, Wang D, Fernandez-Tornero C

Nat Commun (2025) 16 pp. 5254-5254 [ DOI: doi:10.1038/s41467-025-60536-4 Pubmed: 40480971 ]

Sample:
  • Rna polymerase i (Complex from Saccharomyces cerevisiae)
  • Non-template dna (11 kDa, DNA from Saccharomyces cerevisiae)
  • Zinc ion (65 Da, Ligand)
  • Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerase i subunit rpa190 (186 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerase i subunit rpa43 (36 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerase i subunit rpa135 (135 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae)
  • Template dna (11 kDa, DNA from Saccharomyces cerevisiae)
  • Rna (3 kDa, RNA from Saccharomyces cerevisiae)
  • Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerase i subunit rpa49 (46 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerase i subunit rpa34 (26 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerase i complex and rna and dna (Complex)
  • Magnesium ion (24 Da, Ligand)
  • Dna-directed rna polymerase i subunit rpa14 (14 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae)
  • Rna and dna (Complex from Saccharomyces cerevisiae)
  • Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Saccharomyces cerevisiae)
[Show more]
EMD-50956
Download Map
3D View
Yeast RNA polymerase I elongation complex stalled by an apurinic site, 11-subunit
Release date: June 18, 2025
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9g1x
Q-score: 0.479

Santos-Aledo A, Plaza-Pegueroles A, Sanz-Murillo M, Ruiz FM, Hou P, Xu J, Gil-Carton D, Wang D, Fernandez-Tornero C

Nat Commun (2025) 16 pp. 5254-5254 [ DOI: doi:10.1038/s41467-025-60536-4 Pubmed: 40480971 ]

Sample:
  • Template dna (11 kDa, DNA from Saccharomyces cerevisiae)
  • Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae)
  • Rna (3 kDa, RNA from Saccharomyces cerevisiae)
  • Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerase i subunit rpa190 (186 kDa, Protein from Saccharomyces cerevisiae)
  • Non-template dna (11 kDa, DNA from Saccharomyces cerevisiae)
  • Rna and dna (Complex from Saccharomyces cerevisiae)
  • Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerase i complex and rna and dna (Complex)
  • Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae)
  • Zinc ion (65 Da, Ligand)
  • Dna-directed rna polymerase i subunit rpa14 (14 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerase i subunit rpa43 (36 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerase i (Complex from Saccharomyces cerevisiae)
  • Dna-directed rna polymerase i subunit rpa135 (135 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae)
  • Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae)
[Show more]
Sort by: Download
Showing 1 - 2 of 2. Page 1 of 1
Display:
Statistics
×

Download











































Preview first 10
Download

Filter By

Current Database

  • EMDB [2]

Current status

  • REL [2]

Sample type

  • Complex [2]
  • DNA [2]
  • Ligand [2]
  • Protein [2]
  • RNA [2]

Organism

  • Saccharomyces cerevisiae [2]

EM Method

  • Single-particle [2]

Resolution

  • ≥ 2Å and < 3Å [1]
  • ≥ 3Å and < 4Å [1]

Release Date

  • 2025 [2]

Author by name

  • Fernandez-tornero C [2]
  • Gil-carton D [2]
  • Hou P [2]
  • Plaza-pegueroles A [2]
  • Ruiz FM [2]
  • Santos-aledo A [2]
  • Sanz-murillo M [2]
  • Wang D [2]
  • Xu J [2]

Journal

  • NAT COMMUN [2]

Software

  • CRYOLO [1]
  • CRYOSPARC [1]
  • CTFFIND [1]
  • GCTF [1]
  • RELION [1]

Microscope

  • FEI TITAN KRIOS [2]

Electron Source

  • FIELD EMISSION GUN [2]

Film or detector model

  • GATAN K2 SUMMIT (4K X 4K) [2]